by Jeremy W. Dale, Malcolm von Schantz, Nicholas Plant

  • Paperback: 400 pages
  • Publisher: --; 3 edition (December 12, 2011)
  • Language: English
  • ISBN-10: 0470683856
  • ISBN-13: 978-0470683859

The latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation.
The new edition features:

  • Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics.
  • Clear, two-colour diagrams throughout.
  • A dedicated website including all figures.

Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.

Table of Contents

1 Introduction 1
2 Basic Molecular Biology 5
3 How to Clone a Gene 21
4 Purification and Separation of Nucleic Acids 31
5 Cutting and Joining DNA 41
6 Vectors 65
7 Genomic and cDNA Libraries 99
8 Finding the Right Clone 121
9 Polymerase Chain Reaction (PCR) 143
10 DNA Sequencing 161
11 Analysis of Sequence Data 177
12 Analysis of Genetic Variation 209
13 Analysis of Gene Expression 227
14 Analysis of Gene Function 259
15 Manipulating Gene Expression 279
16 Medical Applications, Present and Future 307
17 Transgenics 325
Bibliography 339
Glossary 341
Index 353

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