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Thread: Molecular Cloning: A Laboratory Manual, Third Edition (3 Volume Set) by Joe Sambrook

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    Thumbs up Molecular Cloning: A Laboratory Manual, Third Edition (3 Volume Set) by Joe Sambrook

    «Molecular Cloning: A Laboratory Manual»
    by Joseph Sambrook, David W. Russell

    Pages: 999
    Publisher: CSH Press
    Edition: 2001
    Language: English
    ISBN: 978-087995773


    The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity.

    In this new edition, authors Joe Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology.
    Handsomely redesigned and presented in new bindings of proven durability, this three-volume work is essential for everyone using today’s biomolecular techniques.
    The opening chapters describe essential techniques, some well-established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small.
    These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing.
    The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein-protein interactions.
    The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information.
    As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.


    Chapter 1.
    Plasmids and Their Usefulness in Molecular Cloning
    Chapter 2. Bacteriophage lambda and Its Vectors
    Chapter 3. Working with Bacteriophage M13 Vectors
    Chapter 4. Working with High-capacity Vectors
    Chapter 5. Gel Electrophoresis of DNA and Pulsed-field Agarose Gel Electrophoresis
    Chapter 6. Preparation and Analysis of Eukaryotic Genomic DNA
    Chapter 7. Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
    Chapter 8. In vitro Amplification of DNA by the Polymerase Chain Reaction
    Chapter 9. Preparation of Radiolabeled DNA and RNA Probes
    Chapter 10. Working with Synthetic Oligonucleotide Probes
    Chapter 11. Preparation of cDNA Libraries and Gene Identification
    Chapter 12. DNA Sequencing
    Chapter 13. Mutagenesis
    Chapter 14. Screening Expression Libraries
    Chapter 15. Expression of Cloned Genes in Escherichia coli
    Chapter 16. Introducing Cloned Genes into Cultured Mammalian Cells
    Chapter 17. Analysis of Gene Expression in Cultured Mammalian Cells
    Chapter 18. Protein Interaction Technologies
    Last edited by Motoko; 3rd February 2015 at 12:54 AM. Reason: ADD SUITABLE IMAGE, INFO OF THE BOOK.
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