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    Default Tissue Microarrays: Methods and Protocols

    Tissue Microarrays: Methods and Protocols (Methods in Molecular Biology)
    By Ronald Simon


    •Publisher: Humana Press
    •Number Of Pages: 193
    •Publication Date: 2010-08-19
    •ISBN-10 / ASIN: 1607618052
    •ISBN-13 / EAN: 9781607618058

    Product Description:
    Today’s tissue microarray (TMA) method presents as a modern high-tech technology, one which allows for the linking of clinical data to the tissues that are combined on one slide. In Tissue Microarrays: Methods and Protocols, expert researchers explore the current world of TMA making and TMA applications, providing insight into the inherent and complex aspects of the most popular assays used for in-situ tissue analysis. Chapters examine the range of TMA techniques that allow for a large number of tissues to be included in one TMA, preserve the integrity of donor tissue blocks, and present a highly organized array pattern that allows for the reliable allocation of clinical data to individual tissue spots. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Contemporary and ground-breaking, Tissue Microarrays: Methods and Protocols serves as an essential handbook for pathologists, molecular biologists, researchers in the life sciences, as well as physicians, a reflection of the various applications of current TMA technology.
    Contents
    Preface . v
    Contributors ix
    1 Applications of Tissue Microarray Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
    Ronald Simon
    2 Quality Aspects of TMA Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
    Pierre Tennstedt and Guido Sauter
    3 Representativity of TMA Studies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
    Guido Sauter
    4 Recipient Block TMA Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
    Martina Mirlacher and Ronald Simon
    5 Protocol for Constructing Tissue Arrays by Cutting Edge Matrix Assembly . . . . . 45
    Thai Hong Tran, Justin Lin, Ashley Brooke Sjolund,
    Fransiscus Eri Utama, and Hallgeir Rui
    6 Hypodermic Needle Without Recipient Paraffin Block Technique . . . . . . . . . . . . 53
    Andréa Rodrigues Cordovil Pires and Simone Rabello de Souza
    7 Resin Technologies: Construction and Staining of Resin TMA’s . . . . . . . . . . . . . . 63
    William J. Howat and Susan J. Wilson
    8 Tissue Microarrays from Frozen Tissues-OCT Technique . . . . . . . . . . . . . . . . . . . 73
    Marlena Schoenberg Fejzo and Dennis J. Slamon
    9 An Alternative Technology to Prepare Tissue Microarray Using
    Frozen Tissue Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
    Zhongting Hu, Elbert Chang, and Melissa Hodeib
    10 Building “Tissue” Microarrays from Suspension Cells . . . . . . . . . . . . . . . . . . . . . 93
    Shuchun Zhao and Yasodha Natkunam
    11 Tissue Microarrays from Biopsy Specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
    Milton W. Datta and André A. Kajdacsy-Balla
    12 Immunohistochemical Analysis of Tissue Microarrays . . . . . . . . . . . . . . . . . . . . . . 113
    Ronald Simon, Martina Mirlacher, and Guido Sauter
    13 DNA Copy Number Analysis on Tissue Microarrays . . . . . . . . . . . . . . . . . . . . . . 127
    Anne Kallioniemi
    14 RNA Expression Analysis on Formalin-Fixed Paraffin-Embedded
    Tissues in TMA Format by RNA In Situ Hybridization . . . . . . . . . . . . . . . . . . . . 135
    Jürgen Veeck and Edgar Dahl
    15 Automated Analysis of Tissue Microarrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151


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    Last edited by Micotil; 31st January 2012 at 10:58 PM.
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