• Hello Guest,
    We are experiencing difficulties in sending emails. It related to our platform and how it handles the requests. We are working on it. For now, we are using manual methods in sending emails. Please check your SPAM folder as well. Please let me know if you're still experiencing any issue? Use the Contact US at the bottom of the page to reach out.

    Best regards,
    VETeLiB Admin

Masters Metabolic aspects of oryzanol in rats

whisper

Member
Joined
Jul 1, 2012
Messages
165
Reaction score
16
Points
16
Iraq Professor at Vet School
METABOLIC ASPECTS OF ORYZANOL IN RATS
Michelle Smith Gillespie
Abstract
Using a rat model, this study compared the bioavailability of three forms of
oryzanol, a compound extracted from rice bran oil that has been associated with
improved cholesterol levels. Various methods of extracting lipids from serum and liver
were explored to determine oryzanol absorption. Cholesterol levels were obtained via
enzymatic colorimetric assay and GCMS.
Sprague Dawley retired female breeder rats were sustained for 11 weeks on a
cholesterol- free diet to which either no oryzanol was added (n = 19) or 2.8 g/kg of
oryzanol was added as: 7% oryzanol rice bran oil (RO, n = 8), crystalline oryzanol (CO,
n = 8), or crystalline oryzanol dissolved in tocopherol-stripped corn oil (DO, n = 9). The
percentage of dietary oryzanol recovered in the feces of rats fed the CO diet (41.9 ± 2.21,
mean ± SEM) was significantly higher compared to the RO (28.3 ± 3.54) and DO (27.8 ±
2.63) groups (P < 0.05), suggesting that bioavailability of crystalline oryzanol is lower
relative to the oil forms.
Oryzanol was not detected during GCMS analysis of serum following hexane
extraction. Similarly, hexane extractions of liver analyzed via HPLC and GCMS did not
reveal oryzanol. Hexane may not be an appropriate solvent for extracting oryzanol, or
perhaps the compound is metabolized prior to its entry into the liver or bloodstream.
Pages:87

[thanks-thanks]pdf,245KB,http://depositfiles.com/files/12tu6cnzc[/thanks-thanks]
 
Last edited:
Back
Top