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Molecular Localization Techniques in the Diagnosis and Characterization of Nonhuman Primat

pathvet

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China Veterinary Student
Molecular Localization Techniques in the Diagnosis and Characterization of Nonhuman Primate Infectious Diseases
by K. G. Mansfield, V. G. Sasseville, and S. V. Westmoreland
Veterinary Pathology, 51(1): 110-126
Abstract:
Molecular localization techniques remain important diagnostic and research tools for the pathologist evaluating nonhuman
primate tissues. In situ hybridization and immunohistochemistry protocols have been developed for many important pathogens of
nonhuman primates, including RNA and DNA viruses, prions, and bacterial, protozoal, and fungal pathogens. Such techniques will
remain critical in defining the impact and relevance of novel agents on animal health and disease. A comparative pathology
perspective often provides valuable insight to the best strategy for reagent development and can also facilitate interpretation of
molecular localization patterns. Such a perspective is grounded in a firm understanding of microbe-host pathobiology. This review
summarizes current molecular localization protocols used in the diagnosis of selected primate infectious diseases.
 
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