whisper
Member
- Joined
- Jul 1, 2012
- Messages
- 165
- Reaction score
- 22
- Points
- 16
METABOLIC ASPECTS OF ORYZANOL IN RATS
Michelle Smith Gillespie
Abstract
Using a rat model, this study compared the bioavailability of three forms of
oryzanol, a compound extracted from rice bran oil that has been associated with
improved cholesterol levels. Various methods of extracting lipids from serum and liver
were explored to determine oryzanol absorption. Cholesterol levels were obtained via
enzymatic colorimetric assay and GCMS.
Sprague Dawley retired female breeder rats were sustained for 11 weeks on a
cholesterol- free diet to which either no oryzanol was added (n = 19) or 2.8 g/kg of
oryzanol was added as: 7% oryzanol rice bran oil (RO, n = 8), crystalline oryzanol (CO,
n = 8), or crystalline oryzanol dissolved in tocopherol-stripped corn oil (DO, n = 9). The
percentage of dietary oryzanol recovered in the feces of rats fed the CO diet (41.9 ± 2.21,
mean ± SEM) was significantly higher compared to the RO (28.3 ± 3.54) and DO (27.8 ±
2.63) groups (P < 0.05), suggesting that bioavailability of crystalline oryzanol is lower
relative to the oil forms.
Oryzanol was not detected during GCMS analysis of serum following hexane
extraction. Similarly, hexane extractions of liver analyzed via HPLC and GCMS did not
reveal oryzanol. Hexane may not be an appropriate solvent for extracting oryzanol, or
perhaps the compound is metabolized prior to its entry into the liver or bloodstream.
Pages:87
[thanks-thanks]pdf,245KB,http://depositfiles.com/files/12tu6cnzc[/thanks-thanks]
Michelle Smith Gillespie
Abstract
Using a rat model, this study compared the bioavailability of three forms of
oryzanol, a compound extracted from rice bran oil that has been associated with
improved cholesterol levels. Various methods of extracting lipids from serum and liver
were explored to determine oryzanol absorption. Cholesterol levels were obtained via
enzymatic colorimetric assay and GCMS.
Sprague Dawley retired female breeder rats were sustained for 11 weeks on a
cholesterol- free diet to which either no oryzanol was added (n = 19) or 2.8 g/kg of
oryzanol was added as: 7% oryzanol rice bran oil (RO, n = 8), crystalline oryzanol (CO,
n = 8), or crystalline oryzanol dissolved in tocopherol-stripped corn oil (DO, n = 9). The
percentage of dietary oryzanol recovered in the feces of rats fed the CO diet (41.9 ± 2.21,
mean ± SEM) was significantly higher compared to the RO (28.3 ± 3.54) and DO (27.8 ±
2.63) groups (P < 0.05), suggesting that bioavailability of crystalline oryzanol is lower
relative to the oil forms.
Oryzanol was not detected during GCMS analysis of serum following hexane
extraction. Similarly, hexane extractions of liver analyzed via HPLC and GCMS did not
reveal oryzanol. Hexane may not be an appropriate solvent for extracting oryzanol, or
perhaps the compound is metabolized prior to its entry into the liver or bloodstream.
Pages:87
[thanks-thanks]pdf,245KB,http://depositfiles.com/files/12tu6cnzc[/thanks-thanks]
Last edited: