PCR Protocols & PCR Cloning Protocols

[Tomajwwii]

PCR Cloning Protocols
From Molecular Cloning to Genetic Engineering
by Chen and Janes

Pages: 460
Publisher: Humana Press
Edition: April 1, 2002
Language: English
ISBN: 978-0896039735

Description

Rutgers Univ., New Brunswick, NJ. Covers performing and optimizing PCR, cloning PCR products, mutagenesis and recombination, cloning unknown neighboring DNA, and library construction and screening. Features reproducible methods for isolating genes from all biological samples. Hardcover, comb-bound not yet available.


PCR Protocols
by Stirling and Barlett

Pages: 556
Publisher: Humana Press
Edition: August 1, 2003
Language: English
ISBN-10: 0896036278

Description

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

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[Tomajwwii]

PCR Mutation Detection Protocols
by Theophilus and Rapley

Pages: 305
Publisher: Humana Press
Edition: 2nd., 2011
Language: English
ISBN: 978-1607619468

Description


Since the publication of the popular first edition, the explosion of DNA sequence information, the access to bioinformatics and mutation databases coupled with the ability to readily detect and confirm mutations has cemented the role of molecular diagnostics in medicine and, in particular, mutation detection by the polymerase chain reaction (PCR). In PCR Mutation Detection Protocols, Second Edition, expert researchers bring the subject up-to-date with key protocols involving the PCR and its many various incarnations such as SSCP, CSGE, and dHPLC. The volume also addresses key areas such as Southern blotting, accurate diagnostics with high throughput, as well as microarray systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which provide the often hard to find information that may mean the difference between the success and failure of the method. Authoritative and cutting-edge, PCR Mutation Detection Protocols, Second Edition aims to stimulate postgraduate scientists, researchers, and clinicians already engaged in the area and to provide an important first step for those new to this practice wanting to adopt the powerful and essential technique in their own laboratories.


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PCR Protocols
2nd. Edition, 2003.

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PCR Cloning Protocols
2nd. Edition, 2002

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