Wild Bird HPAI Surveillance: sample collection from healthy, sick and dead birds

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Wild Bird HPAI Surveillance: sample collection from healthy, sick and dead birds
By Karrie Rose, Scott Newman, Marcela Uhart, Juan Lubroth



Pages: 55
Publisher: FAO
Edition: 1st ed., 2006
Language: English
ISBN: 92-5-000000-0

Description

Contents
Introduction 1
CHAPTER 1
Clinical signs of infectious disease 3
CHAPTER 2
Live bird handling 5
Blood sampling 5
Euthanasia 6
CHAPTER 3
Dead bird collection 9
Sampling strategy for H5N1 AI 10
CHAPTER 4
Avian Necropsy Protocol 13
Necropsy Occupational Health and Safety 13
Avian Necropsy Protocol 14
External examination 14
Internal Examination 15
CHAPTER 5
Necropsy sample collection 21
CHAPTER 6
Sample collection 23
Details regarding samples to be collected during a necropsy 23
CHAPTER 7
Swabbing techniques 25
Procedure for sampling 26
CHAPTER 8
Sample handling and transport 29
Swabs and viral transport media 29
Serum, plasma and fresh tissues 29
Formalin fixed tissues 30
Sample shipment 30
CHAPTER 9
Diagnostics 33
Laboratory diagnosis for H5N1 AI 33
Field tests (point-of-care) 34
RT-PCR 35
CHAPTER 10
Carcass disposal 37
In the field 37
CHAPTER 11
Disinfection 39
CHAPTER 12
Personal safety recommendations 41
Annex 1: Sick or dead bird sample collection log 45

Annex 2: OIE/FAO Network (OFFLU) and reference laboratories
for avian influenza 47
Annex 3: Gross Pathology Illustrations 51

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